Nanobody, also called as Single Domain Antibody. A single-domain antibody (sdAb) is an antibody fragment consisting of a single monomeric variable antibody domain. It is able to bind selectively to a specific antigen like a whole antibody.
With a molecular weight of only 12–15 kDa, single-domain antibodies are much smaller than common antibodies (150–160 kDa) which are composed of two heavy protein chains and two light chains, and even smaller than Fab fragments (~50 kDa, one light chain and half a heavy chain) and single-chain variable fragments (~25 kDa, two variable domains, one from a light and one from a heavy chain).
The main disadvantage of nanobodies is their short half-life as drugs. There have been many methods that can be effectively modified to increase the half-life, including methods such as Fc fusion, PEGylation, albumin fusion, and multivalent nanobody fusion.
Llama Single-domain Antibody
Single Domain Ab
Single Domain Antibody
Single Domain/SdAb or VHH Antibody
Single Domain Antibody(sdAb)
Immunization of llama on standard 12 weeks protocol
ELISA using each bleed against antigen
|Phase II||PBMCs isolation||PBMC's isolated and preserved from 100mL blood collected at weeks 8 and 12||Inquiry|
|Phase III||Library Construction||sdAb library construction from Phase II PBMC's||Inquiry|
|Phase IV||Library Screening||
Screening by direct ELISA
Selection and sequencing of high affinity binders
Relative affinity determination by ELISA
|Phase V||Production||Express and purification of additional quantities of antibody||Inquiry|